X-Ray Synchrotron Radiation Pseudo-Holotomography:
a New Technique for Blood Vess el 3D Visualization

Mastrogiacomo Maddalena¹, Komlev Vladimir S.², Peyrin Francoise³, Rustichelli Franco², Cancedda Ranieri¹

1) Istituto Nazionale per la Ricerca sul Cancro, Dipartimento di Oncologia, Genova, Italy;
2) Universitè Politecnica delle Marche, Dip.SAIFET, Ancona, Italy;
3) European Synchrotron Radiation Facility Grenoble and CREATIS, Lyon, France

Нанотехнологии в диагностике, хирургии, робототехнике
Конференция «Нанотехнологии в онкологии 2009»

Standard X-ray micro-computed tomography is a technique that allows a good visualization of the structure of mineralized tissues and biomaterials, but it fails to finely discern soft tissues. The X-ray synchrotron radiation pseudo-holotomography was used to visualize, at three-dimensional (3D) level, microvascular networks for the first time with no need for contrast agents, and to extract quantitative structural data in a boneengineered construct. The pseudo-holotomography is an innovative technique that offers a promising powerful tool to investigate angio and microvasculogenesis in advanced biomedical research areas such as regenerative medicine and antiangiogenic cancer therapies [1].

Reference

1. Komlev V.S., Mastrogiacomo M., Peyrin F., Cancedda R. and Rustichelli F. "X-Ray Synchrotron Radiation Pseudo-Holotomography as a New Imaging Technique to Investigate Angio- and Microvasculogenesis with No Usage of Contrast Agents" Tissue Engineering Part C: Methods September 2009, Vol.15, No.3: 425-430.